| Gene | Leng1 |
| Chromosome | 7 |
| Deletion Start | 3,665,444 |
| Deletion End | 3,661,129 |
| Deletion Size | 4,316 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,664,861 | 3,664,881 | TTGGAACAGGGTCTGTGTAGC |
| TUP | 3,664,834 | 3,664,860 | CGTGGCTGTTATGGAACTGGCTACGTA |
| TUR | 3,664,805 | 3,664,826 | GGCAAGCTGAGTTCAAGACCAG |
| TDF | 3,661,224 | 3,661,246 | GGTGGAAGCAGAAGAGACAGATG |
| TDP | 3,661,199 | 3,661,221 | CGGAGGCGACGATACAACTCCCA |
| TDR | 3,661,143 | 3,661,162 | AGTGGGCAGGAGTTGGGTTC |
PCR Primers
| Primer | Sequence |
| SU | TAAACAACAGTCAAGGCCGC |
| SD | TCCCTCGCATGCTGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16699A-G1 | VGB6 | C57BL/6NTac | |
| 16699A-H12 | VGB6 | C57BL/6NTac | |
