| Gene | Nhlrc4 |
| Chromosome | 17 |
| Deletion Start | 25,943,768 |
| Deletion End | 25,943,265 |
| Deletion Size | 504 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,943,659 | 25,943,681 | TTTGCTGTGAGTGAAGAGTTTGG |
| TUP | 25,943,629 | 25,943,650 | AGCTGTTTGGCAGTGCCCACCA |
| TUR | 25,943,595 | 25,943,612 | ATGCCCAGTCAGGGTTCC |
| TDF | 25,943,461 | 25,943,482 | TGCAGTTGGAGGGACTAAAGAG |
| TDP | 25,943,437 | 25,943,459 | CCCTTGGGTATGGCCTGTGCACC |
| TDR | 25,943,397 | 25,943,414 | GCAGTTGTCCCCTGCATC |
PCR Primers
| Primer | Sequence |
| SU | CCTGACACCTTGACCTCCTG |
| SD | CTAAGGGACCACACCCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16700A-A4 | VGB6 | C57BL/6NTac | |
| 16700A-A10 | VGB6 | C57BL/6NTac | |
| 16700A-C10 | VGB6 | C57BL/6NTac | |
| 16700A-E3 | VGB6 | C57BL/6NTac | |
| 16700A-E11 | VGB6 | C57BL/6NTac | |
