| Gene | Pin4 |
| Chromosome | X |
| Deletion Start | 102,127,733 |
| Deletion End | 102,119,525 |
| Deletion Size | 8,209 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,119,767 | 102,119,789 | TCAGGGTATCCATGAGGTTTCAG |
| TUP | 102,119,797 | 102,119,818 | ACCCTCGTGGCGGGCAATAGAA |
| TUR | 102,119,828 | 102,119,847 | CCAGGTCACGAGGAACCATC |
| TDF | 102,127,301 | 102,127,321 | GCCTGCTCCATCCAGTCTTAG |
| TDP | 102,127,322 | 102,127,343 | ACGGCCTCCTGCCATTTGTCCC |
| TDR | 102,127,350 | 102,127,372 | CATCTGGCTATGTAGGCATTGTC |
PCR Primers
| Primer | Sequence |
| SU | CTTTCTCGTCTCTGGGCAAC |
| SD | GAGAGCGAACGTTAAATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16703A-B9 | VGB6 | C57BL/6NTac | |
| 16703A-C3 | VGB6 | C57BL/6NTac | |
| 16703A-E2 | VGB6 | C57BL/6NTac | |
| 16703A-E12 | VGB6 | C57BL/6NTac | |
| 16703A-F5 | VGB6 | C57BL/6NTac | |
| 16703A-F6 | VGB6 | C57BL/6NTac | |
