| Gene | Nlgn1 |
| Chromosome | 3 |
| Deletion Start | 26,133,731 |
| Deletion End | 26,133,246 |
| Deletion Size | 486 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,133,646 | 26,133,663 | ATCCGGTGCCCCATTGAC |
| TUP | 26,133,619 | 26,133,645 | TCTCTGCTTGTTGGGATGTTTGCTACA |
| TUR | 26,133,577 | 26,133,601 | GGGTCTACATCATCCAACTTTTGAG |
| TDF | 26,133,428 | 26,133,447 | GCCTCCAGAACCACCATCTC |
| TDP | 26,133,404 | 26,133,426 | CTGGTCTGACATCCGGAACGCCA |
| TDR | 26,133,351 | 26,133,372 | TTCAGGCAATCTGCCATCAATG |
PCR Primers
| Primer | Sequence |
| SU | AGACCCACTGCTACCTGG |
| SD | CCTGCACATGCAATGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16704A-A3 | VGB6 | C57BL/6NTac | |
| 16704A-C2 | VGB6 | C57BL/6NTac | |
| 16704A-H4 | VGB6 | C57BL/6NTac | |
| 16704A-D12 | VGB6 | C57BL/6NTac | |
| 16704A-D2 | VGB6 | C57BL/6NTac | |
| 16704A-F12 | VGB6 | C57BL/6NTac | |
