| Gene | Ptpn14 |
| Chromosome | 1 |
| Deletion Start | 189,798,677 |
| Deletion End | 189,786,778 |
| Deletion Size | 11,900 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 189,787,645 | 189,787,664 | CGCAGTCCTCTGCTTGAATG |
| TUP | 189,787,668 | 189,787,695 | AGCACTTACTGCCGTAGCTGTCTTCACA |
| TUR | 189,787,703 | 189,787,727 | CAAATGCCTATCTTAACAGCTCTCC |
| TDF | 189,798,515 | 189,798,536 | CACTACTTCGGCCTTTGGTTTC |
| TDP | 189,798,538 | 189,798,557 | CAGCAAGAGCCAGCAGGCGA |
| TDR | 189,798,563 | 189,798,583 | CAGTGGCTTCTCCAGCTCTAC |
PCR Primers
| Primer | Sequence |
| SU | CCTGAGTCTGCTCCAATG |
| SD | CAGAATTAGGTGGCTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16705A-A12 | VGB6 | C57BL/6NTac | |
| 16705A-F4 | VGB6 | C57BL/6NTac | |
| 16705A-F7 | VGB6 | C57BL/6NTac | |
| 16705A-G6 | VGB6 | C57BL/6NTac | |
