| Gene | Ncoa1 |
| Chromosome | 12 |
| Deletion Start | 4,344,482 |
| Deletion End | 4,322,872 |
| Deletion Size | 21,611 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,344,432 | 4,344,455 | CCTGCAAACCCAGACTCTCATAAG |
| TUP | 4,344,403 | 4,344,428 | AAAGGATCGCCATGTGACACGCTGGC |
| TUR | 4,344,380 | 4,344,400 | ACAGAGCGCATTCCTACCTTG |
| TDF | 4,323,847 | 4,323,869 | CGGCTCCTGTTCATTCCACTAAG |
| TDP | 4,323,806 | 4,323,835 | CTGCAGGCCTCATTGTACTTTGTACTCAGC |
| TDR | 4,323,777 | 4,323,796 | GGCACCTGGCAAGGCATAAG |
PCR Primers
| Primer | Sequence |
| SU | TTACATTTATACATGCAAGTG |
| SD | AGAACTTACACTTCAGTTACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16711A-A5 | VGB6 | C57BL/6NTac | |
| 16711A-F7 | VGB6 | C57BL/6NTac | |
| 16711A-C10 | VGB6 | C57BL/6NTac | |
| 16711A-F5 | VGB6 | C57BL/6NTac | |
| 16711A-G9 | VGB6 | C57BL/6NTac | |
| 16711A-D5 | VGB6 | C57BL/6NTac | |
