| Gene | Pcdh9 |
| Chromosome | 14 |
| Deletion Start | 93,888,729 |
| Deletion End | 93,885,700 |
| Deletion Size | 3,030 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,887,998 | 93,888,020 | TCCAGGTCACAGTAAGTGATGTC |
| TUP | 93,887,965 | 93,887,993 | ACAACAGGCCAGTGTTTAAAGAGGGTCAA |
| TUR | 93,887,901 | 93,887,920 | CAGTGGCATGGAGCTGAATG |
| TDF | 93,886,502 | 93,886,522 | AGAAGAGAAGCCAGCACCTAC |
| TDP | 93,886,481 | 93,886,501 | TGATGTGGGCCTGCACCGTCT |
| TDR | 93,886,425 | 93,886,445 | CCAGCACGAGAGTGTGCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GCAAATGCAACAAGCAGG |
| SD | CCAGGAGCAGAATTGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16712A-A1 | VGB6 | C57BL/6NTac | |
| 16712A-A3 | VGB6 | C57BL/6NTac | |
| 16712A-A8 | VGB6 | C57BL/6NTac | |
