| Gene | Rasl2-9 |
| Chromosome | 7 |
| Deletion Start | 5,125,926 |
| Deletion End | 5,125,279 |
| Deletion Size | 648 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,125,852 | 5,125,872 | GGCACCGGAAAGACGACATTC |
| TUP | 5,125,829 | 5,125,850 | TGAAGCGCCACTTGACCGGAGA |
| TUR | 5,125,805 | 5,125,828 | GTGGCTACATACTCCTTCTCAAAC |
| TDF | 5,125,521 | 5,125,544 | TGAAAGTGAAGGCAAAACCTATTC |
| TDP | 5,125,497 | 5,125,519 | CTTCCACCGAAAGAAGAATCTTC |
| TDR | 5,125,419 | 5,125,440 | CTCCAATGAGCTTTCTGGCAAG |
PCR Primers
| Primer | Sequence |
| SU | GTCATGAATCCTTGATTCGG |
| SD | TTCACCACTGACTATTGTGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16714A-B1 | VGB6 | C57BL/6NTac | |
| 16714A-A6 | VGB6 | C57BL/6NTac | |
| 16714A-A8 | VGB6 | C57BL/6NTac | |
