| Gene | Gm5465 |
| Chromosome | 14 |
| Deletion Start | 79,453,596 |
| Deletion End | 79,451,870 |
| Deletion Size | 1,727 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,451,874 | 79,451,893 | CCGTCGGCTACGCGATAAAG |
| TUP | 79,451,902 | 79,451,920 | CTCCGCCCCGAAGGCCCAA |
| TUR | 79,451,948 | 79,451,965 | AGCGGCGACCCAGAACAG |
| TDF | 79,453,394 | 79,453,413 | GGTGGACGAGACCCTGTTAC |
| TDP | 79,453,414 | 79,453,442 | TGGAGCTGAATTAACAGATGTGGGACGCT |
| TDR | 79,453,444 | 79,453,465 | CCTCCTGTTCTTCTGGACACTG |
PCR Primers
| Primer | Sequence |
| SU | TTCCAAAGCAATGCAGCTC |
| SD | CTTCAGCCAGGAAACTGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16718A-C5 | VGB6 | C57BL/6NTac | |
| 16718A-G12 | VGB6 | C57BL/6NTac | |
| 16718A-E1 | VGB6 | C57BL/6NTac | |
| 16718A-G1 | VGB6 | C57BL/6NTac | |
| 16718A-G6 | VGB6 | C57BL/6NTac | |
| 16718A-D7 | VGB6 | C57BL/6NTac | |
