| Gene | Rfwd3 |
| Chromosome | 8 |
| Deletion Start | 111,300,177 |
| Deletion End | 111,273,011 |
| Deletion Size | 27,167 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,299,822 | 111,299,844 | TGACTGACTCTTTGACCCTTGTC |
| TUP | 111,299,783 | 111,299,805 | TTTATCACCGCAGCGGAACTGCG |
| TUR | 111,299,748 | 111,299,767 | CCCGCAGGAAGAGGAGAATG |
| TDF | 111,273,361 | 111,273,381 | GTTCCACGAGGACAGAGGTAG |
| TDP | 111,273,331 | 111,273,360 | TGGCCTTGTCTTTAGAAGGGACAGTATGTA |
| TDR | 111,273,282 | 111,273,305 | AAAAGCAAGCCAAGAACTCATTTC |
PCR Primers
| Primer | Sequence |
| SU | GCCGAACAGTTCAAAGGAAG |
| SD | ATCCAAGAAGCAAGGCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16723A-C7 | VGB6 | C57BL/6NTac | |
| 16723A-D9 | VGB6 | C57BL/6NTac | |
| 16723A-G12 | VGB6 | C57BL/6NTac | |
| 16723A-G4 | VGB6 | C57BL/6NTac | |
| 16723A-H9 | VGB6 | C57BL/6NTac | |
