16727   Acrbp     Status: ES cell colonies screened / QC positives

GeneAcrbp
Chromosome6
Deletion Start125,063,083
Deletion End125,050,013
Deletion Size13,071
Genome Build38
MGI IDMGI:1859515
NCBI Gene ID54137
BAC ID295I1
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,050,859125,050,880CCGATCTCCCTTATGCTTCCTG
TUP125,050,882125,050,907TTTGAGTCCTTCTGCCAGTTTGCTCA
TUR125,050,946125,050,967GGATCACCAGATGCAACCTTTC
TDF125,062,489125,062,507GCAGTGCCTGCTGAGAAAC
TDP125,062,508125,062,534CAGAACAGAAAGGTGAGCTGCTGACCC
TDR125,062,536125,062,554CACTAAGGGCGGTGTGGAC

PCR Primers

PrimerSequence
SUGGAAGTAGCGCGCAGACC
SDCCTTTAGGTGCAGCTCCTTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR109 bp++-
TDFTDR66 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
16727A-A11VGB6C57BL/6NTac
16727A-B11VGB6C57BL/6NTac
16727A-C9VGB6C57BL/6NTac
16727A-D5VGB6C57BL/6NTac
16727A-G10VGB6C57BL/6NTac
16727A-G5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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