| Gene | Dspp |
| Chromosome | 5 |
| Deletion Start | 104,178,641 |
| Deletion End | 104,174,030 |
| Deletion Size | 4,612 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,174,631 | 104,174,653 | GGGTTGTTCCTGTACTTGAGAAG |
| TUP | 104,174,657 | 104,174,682 | TGTAAGCACTCCGAGCTCACTCTTGC |
| TUR | 104,174,712 | 104,174,733 | TGAGAGTGGACCACTGGAGAAG |
| TDF | 104,178,466 | 104,178,486 | TGACAGCAAGGATAGCAGTTC |
| TDP | 104,178,487 | 104,178,510 | TGACAGCAGTGATGGTGACAGCAA |
| TDR | 104,178,511 | 104,178,531 | TCACTGTTGCCATTACCAGAC |
PCR Primers
| Primer | Sequence |
| SU | AGGAGAGACGTTACCCTC |
| SD | AAGACTAGCTCTCTGGTGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16738A-F11 | VGB6 | C57BL/6NTac | |
| 16738A-G3 | VGB6 | C57BL/6NTac | |
