| Gene | BC024479 |
| Chromosome | 9 |
| Deletion Start | 37,523,580 |
| Deletion End | 37,489,327 |
| Deletion Size | 34,254 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,490,132 | 37,490,150 | AACTGTCGCGGGTGCTGTC |
| TUP | 37,490,152 | 37,490,177 | CCCGCACTGCTTTGTTCCCAGATAGA |
| TUR | 37,490,208 | 37,490,230 | TGCTACCCTTCCTCAATTCCATC |
| TDF | 37,523,230 | 37,523,252 | CCTCTCAGCACAAGCCTCATTAC |
| TDP | 37,523,256 | 37,523,276 | TGGAAGTGGAACCCACCCGGA |
| TDR | 37,523,278 | 37,523,301 | GCAATACCGAGGTAGCAGTAGATG |
PCR Primers
| Primer | Sequence |
| SU | GCCAATTCGTCCTTAATTCG |
| SD | CTGTGACTTGATTTACTCGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16740A-A8 | VGB6 | C57BL/6NTac | |
| 16740A-G10 | VGB6 | C57BL/6NTac | |
