| Gene | Slc25a36 |
| Chromosome | 9 |
| Deletion Start | 97,110,948 |
| Deletion End | 97,078,997 |
| Deletion Size | 31,952 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 97,110,377 | 97,110,396 | CTTCAGCACTGCCTGCTTTG |
| TUP | 97,110,337 | 97,110,366 | ATGGATGGTTTGAGAGAAGGCGACTACTAA |
| TUR | 97,110,290 | 97,110,311 | GACTTCGGGCTCCACACTAATC |
| TDF | 97,079,536 | 97,079,557 | AGTCTGACTGGTCTCAGGAAGC |
| TDP | 97,079,506 | 97,079,527 | CACTGAATCACTGTCATGAGTT |
| TDR | 97,079,468 | 97,079,487 | CTCCTCCTTACCGAGCACAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTAGATCGTCAAGAGCCG |
| SD | TGCTCATGGAATCAACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16750A-B5 | VGB6 | C57BL/6NTac | |
| 16750A-G6 | VGB6 | C57BL/6NTac | |
| 16750A-E10 | VGB6 | C57BL/6NTac | |
