| Gene | Fxc1 |
| Chromosome | 7 |
| Deletion Start | 105,641,282 |
| Deletion End | 105,640,596 |
| Deletion Size | 687 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,640,726 | 105,640,744 | TAGCGGTGGTGAAGTCGTG |
| TUP | 105,640,753 | 105,640,780 | TTCTCTCAGTTGCGAGACTTCCTGTTGG |
| TUR | 105,640,796 | 105,640,815 | GCGCTGGAAACACAGTTCTG |
| TDF | 105,640,998 | 105,641,020 | AGTTTCTACTTCCCTCCGTCTTC |
| TDP | 105,641,021 | 105,641,042 | CTTCCAGGAGGCCTGCCTGCAC |
| TDR | 105,641,045 | 105,641,065 | TGGATGAGTTTCCCAGCACAG |
PCR Primers
| Primer | Sequence |
| SU | TCGTCGCGATCCTAGCAG |
| SD | TTAGTCCCAAGGACAGCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16754A-B4 | VGB6 | C57BL/6NTac | |
| 16754A-B9 | VGB6 | C57BL/6NTac | |
| 16754A-B12 | VGB6 | C57BL/6NTac | |
| 16754A-C1 | VGB6 | C57BL/6NTac | |
| 16754A-D5 | VGB6 | C57BL/6NTac | |
| 16754A-H4 | VGB6 | C57BL/6NTac | |
