| Gene | Tm2d1 |
| Chromosome | 4 |
| Deletion Start | 98,383,233 |
| Deletion End | 98,357,868 |
| Deletion Size | 25,366 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,383,062 | 98,383,082 | TCAGGGTGGGACAATATCCTC |
| TUP | 98,383,037 | 98,383,058 | AAAGCCGCCGTTCTCAGGCGGT |
| TUR | 98,382,974 | 98,382,995 | CCAATGGTCACTAGAGCCACAG |
| TDF | 98,358,360 | 98,358,382 | GGAAAAGGCTGAGGTGGGAATAC |
| TDP | 98,358,336 | 98,358,358 | TGGTTCAGGCATTGCCAGCCTGC |
| TDR | 98,358,313 | 98,358,332 | TATGCTCTGCTCCCGTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CTAGAGAAGACTCCCAAACGTG |
| SD | AGGCACTTCTCACAGAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16766A-E12 | VGB6 | C57BL/6NTac | |
| 16766B-G3 | VGB6 | C57BL/6NTac | |
| 16766C-E12 | VGB6 | C57BL/6NTac | |
