| Gene | Rwdd3 |
| Chromosome | 3 |
| Deletion Start | 121,171,673 |
| Deletion End | 121,171,026 |
| Deletion Size | 648 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,171,592 | 121,171,613 | TGGGAGATGTTGAGCTGTTCAG |
| TUP | 121,171,567 | 121,171,590 | TGAATACCCTGCCTCTCACCCTGC |
| TUR | 121,171,521 | 121,171,538 | TGTCCAGGGCCACAAGAC |
| TDF | 121,171,153 | 121,171,173 | AAATGGCAGCCGATTCCAGAC |
| TDP | 121,171,132 | 121,171,152 | TTCTGCTGGACGCAGCATGGA |
| TDR | 121,171,107 | 121,171,128 | CCTGCCTGGGTTAGAAGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTAGCCTGGTGAAGCAC |
| SD | GTCTTCCTAGAGCTAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16769A-D6 | VGB6 | C57BL/6NTac | |
| 16769A-E2 | VGB6 | C57BL/6NTac | |
| 16769A-F4 | VGB6 | C57BL/6NTac | |
| 16769A-E9 | VGB6 | C57BL/6NTac | |
