| Gene | Hoxa4 |
| Chromosome | 6 |
| Deletion Start | 52,191,685 |
| Deletion End | 52,190,297 |
| Deletion Size | 1,389 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,191,083 | 52,191,100 | GCTTTGGCTGTGGGTGTG |
| TUP | 52,191,056 | 52,191,075 | CGCCCGCAGCCTGGTCCTAA |
| TUR | 52,190,999 | 52,191,020 | TTTGGCGTCTCGTAAATCTCAG |
| TDF | 52,190,553 | 52,190,571 | GCATCGAGATCGCCCACAC |
| TDP | 52,190,529 | 52,190,551 | CTCTGCTTGTCGGAGCGCCAGGT |
| TDR | 52,190,494 | 52,190,515 | TCCACTTCATTCTCCGGTTCTG |
PCR Primers
| Primer | Sequence |
| SU | CCGAGGAACCCAAGCTCG |
| SD | GGCCTGCTTCTTCTGCATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16779A-D9 | VGB6 | C57BL/6NTac | |
| 16779A-E10 | VGB6 | C57BL/6NTac | |
| 16779A-F11 | VGB6 | C57BL/6NTac | |
