| Gene | Ralgps1 |
| Chromosome | 2 |
| Deletion Start | 33,340,766 |
| Deletion End | 33,324,656 |
| Deletion Size | 16,111 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,339,879 | 33,339,898 | TAGCCCAGTGGATGCTGTTC |
| TUP | 33,339,853 | 33,339,878 | TCTAGGAGGCCAGAGTAGTTCCACGG |
| TUR | 33,339,820 | 33,339,840 | GCAGTGAGGAGGACGACTTTG |
| TDF | 33,325,320 | 33,325,341 | AGCCCAGCATTGGCTATATCTC |
| TDP | 33,325,274 | 33,325,303 | TATACTTCTGCTTGTTGAAGCCCTGTCTGC |
| TDR | 33,325,229 | 33,325,250 | ACACGAATGGTGTATCCACTCC |
PCR Primers
| Primer | Sequence |
| SU | TGCTACCAGAAGGGCTATGG |
| SD | GAGGTTTGCTGTGGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16787A-A1 | VGB6 | C57BL/6NTac | |
| 16787A-D12 | VGB6 | C57BL/6NTac | |
| 16787A-H3 | VGB6 | C57BL/6NTac | |
| 16787A-C7 | VGB6 | C57BL/6NTac | |
| 16787A-E9 | VGB6 | C57BL/6NTac | |
| 16787A-G12 | VGB6 | C57BL/6NTac | |
