| Gene | Kti12 |
| Chromosome | 4 |
| Deletion Start | 108,849,010 |
| Deletion End | 108,847,894 |
| Deletion Size | 1,117 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,848,118 | 108,848,139 | CATCCTGGACTCGGTGAACTAC |
| TUP | 108,848,142 | 108,848,165 | CAAGGGCTTCCGCTACGAGCTGTA |
| TUR | 108,848,196 | 108,848,216 | CGCACGCAGTAAAGTAAGCAG |
| TDF | 108,848,684 | 108,848,703 | GCAGCTTTCTGCACCAGTTG |
| TDP | 108,848,706 | 108,848,728 | TCAGGCCACAAGCCAGGTGTTGA |
| TDR | 108,848,734 | 108,848,754 | GCTCTTCTGGGCTTCCATCAC |
PCR Primers
| Primer | Sequence |
| SU | CCAACCATGAAAGGATGTTG |
| SD | CAGTTTCCCAGGGACAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16791A-E8 | VGB6 | C57BL/6NTac | |
| 16791A-F8 | VGB6 | C57BL/6NTac | |
| 16791A-G4 | VGB6 | C57BL/6NTac | |
| 16791A-C1 | VGB6 | C57BL/6NTac | |
