16795   Dnajc30     Status: ES cell colonies screened / QC positives

GeneDnajc30
Chromosome5
Deletion Start135,065,002
Deletion End135,064,254
Deletion Size749
Genome Build38
MGI IDMGI:1913364
NCBI Gene ID66114
BAC ID87E19
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,064,542135,064,562ATCCTCCGGCGCAAGTATGAC
TUP135,064,565135,064,587AGGTTTGCTCAGCGACCAGGACC
TUR135,064,594135,064,611AGGGCTTGACGCCAGGTC
TDF135,064,704135,064,724CGCACCATGTTCGACTTTGAC
TDP135,064,728135,064,750TTCTACCAGGCGCACTACGGAGA
TDR135,064,758135,064,776GCAAACGCCGTTCTCTCTC

PCR Primers

PrimerSequence
SUTATTTCCGGGCTTCATTCTG
SDCAAATCTTGCTCAGGGCTTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR70 bp++-
TDFTDR73 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
16795A-A9VGB6C57BL/6NTac
16795A-F1VGB6C57BL/6NTac
16795A-H2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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