| Gene | 1810035L17Rik |
| Chromosome | 12 |
| Deletion Start | 87,449,939 |
| Deletion End | 87,443,933 |
| Deletion Size | 6,007 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,444,343 | 87,444,365 | AACTCTTTAGGCACGATCCTCTC |
| TUP | 87,444,366 | 87,444,389 | CACTCGCCCGATCGACAGTAGTCA |
| TUR | 87,444,428 | 87,444,450 | TGACTCGTGAGTGTCCAAGTAAG |
| TDF | 87,449,504 | 87,449,524 | CCAGGGACTGAGTGCAGAGTG |
| TDP | 87,449,525 | 87,449,546 | CACGCGTATGCTAAGCCCAGGC |
| TDR | 87,449,582 | 87,449,603 | ACATACGTGCTGATACGCCATC |
PCR Primers
| Primer | Sequence |
| SU | AAGTGCGCCTCTGAGAAGTC |
| SD | CTCGATGATAGATCCCAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16802A-B12 | VGB6 | C57BL/6NTac | |
| 16802A-D9 | VGB6 | C57BL/6NTac | |
| 16802A-E5 | VGB6 | C57BL/6NTac | |
| 16802A-H6 | VGB6 | C57BL/6NTac | |
| 16802A-E8 | VGB6 | C57BL/6NTac | |
