| Gene | Tnfrsf13c |
| Chromosome | 15 |
| Deletion Start | 82,224,309 |
| Deletion End | 82,223,043 |
| Deletion Size | 1,267 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,224,239 | 82,224,258 | CCCACCCAGTGCAATCAGAC |
| TUP | 82,224,216 | 82,224,238 | CGAGTGCTTCGACCCTCTGGTGA |
| TUR | 82,224,197 | 82,224,215 | TCACAGGACACGCAGTTTC |
| TDF | 82,223,486 | 82,223,508 | CAAGGGCCTGATGCTAGGTTATG |
| TDP | 82,223,463 | 82,223,484 | CCTGTGGGCTGACCAGACACCC |
| TDR | 82,223,418 | 82,223,438 | GGAAGTGCTGAGTTCAGAGAC |
PCR Primers
| Primer | Sequence |
| SU | ACAATTGGTCTTGGTGAGGC |
| SD | GAGATTAAGTTTCCAGTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16811A-A12 | VGB6 | C57BL/6NTac | |
| 16811A-B1 | VGB6 | C57BL/6NTac | |
| 16811A-B12 | VGB6 | C57BL/6NTac | |
| 16811A-B6 | VGB6 | C57BL/6NTac | |
