| Gene | Jph1 |
| Chromosome | 1 |
| Deletion Start | 17,097,601 |
| Deletion End | 17,091,302 |
| Deletion Size | 6,300 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,096,906 | 17,096,927 | CACTGTCACTTGAGCCTGTCAC |
| TUP | 17,096,875 | 17,096,900 | ATTCCTGATCCCTCCCTCTCCAGGCC |
| TUR | 17,096,853 | 17,096,872 | CCGGAGTGGCGATTTCAAAC |
| TDF | 17,091,748 | 17,091,767 | CGCAAGTCCGAATCCAAGTC |
| TDP | 17,091,725 | 17,091,747 | TTCCATCTCCAGCAAGCGCAGCT |
| TDR | 17,091,697 | 17,091,715 | CTGCTCATGGCTGCATCAC |
PCR Primers
| Primer | Sequence |
| SU | TCTGGAGACTGGGCTGTC |
| SD | GATTTCACCTAGAAAGCTTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16815A-F9 | VGB6 | C57BL/6NTac | |
| 16815A-A5 | VGB6 | C57BL/6NTac | |
