| Gene | Sycn |
| Chromosome | 7 |
| Deletion Start | 28,542,124 |
| Deletion End | 28,540,913 |
| Deletion Size | 1,212 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,541,202 | 28,541,220 | GCAAGACCCGCAAGTTCAC |
| TUP | 28,541,221 | 28,541,241 | CGCGGGTTCTTACCCTCGCCT |
| TUR | 28,541,281 | 28,541,301 | CAGTAGAGGGCAGAGATGGAG |
| TDF | 28,541,839 | 28,541,859 | CCAGGCTCATTCCTAGTGGTC |
| TDP | 28,541,860 | 28,541,883 | CCCAGGTCCAGCAATCGTCCCAAG |
| TDR | 28,541,887 | 28,541,908 | CAGAGGATACCCGAGGTCTGTG |
PCR Primers
| Primer | Sequence |
| SU | CCTAGACTGTCTTTAACCAG |
| SD | CTGTGGTTCTACCCTGGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16817A-A4 | VGB6 | C57BL/6NTac | |
| 16817A-A10 | VGB6 | C57BL/6NTac | |
| 16817A-B1 | VGB6 | C57BL/6NTac | |
| 16817A-F12 | VGB6 | C57BL/6NTac | |
| 16817A-B2 | VGB6 | C57BL/6NTac | |
| 16817A-B10 | VGB6 | C57BL/6NTac | |
