| Gene | Wscd2 |
| Chromosome | 5 |
| Deletion Start | 113,551,585 |
| Deletion End | 113,550,938 |
| Deletion Size | 648 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,550,949 | 113,550,970 | GTTCAAGTTCCAGCGGTGTTTC |
| TUP | 113,550,974 | 113,550,996 | TATTTCCGCAGGAAGCCGGTGCG |
| TUR | 113,551,014 | 113,551,034 | TCCCAGCGGTCAGGTAGATTG |
| TDF | 113,551,502 | 113,551,522 | CCCACACTTGCCTTGGTTTAG |
| TDP | 113,551,525 | 113,551,552 | TCCCACTGCTTGTTAAACACACAGCAAC |
| TDR | 113,551,555 | 113,551,574 | GCCCTGGAACCCTGATTCTG |
PCR Primers
| Primer | Sequence |
| SU | GTTCTTTAGCCACACTCTG |
| SD | TCACCAGGGCAGTGTTAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16819A-B11 | VGB6 | C57BL/6NTac | |
| 16819A-D10 | VGB6 | C57BL/6NTac | |
| 16819A-H5 | VGB6 | C57BL/6NTac | |
| 16819A-G1 | VGB6 | C57BL/6NTac | |
| 16819A-C2 | VGB6 | C57BL/6NTac | |
