| Gene | Trmt112 |
| Chromosome | 19 |
| Deletion Start | 6,910,954 |
| Deletion End | 6,910,188 |
| Deletion Size | 767 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CAATCTCCTAAGCTCCCATGTGC |
| TUP | | | ACGCGTGGCTTCCCGCTGCG |
| TUR | | | ATGGGCGATGAACCCTTGTG |
| TDF | | | CAGACCTCCCACTGAGGCTAC |
| TDP | | | CGGTGCTTAGCTTTGTCCTGCTCCC |
| TDR | | | GGTGCCCTCCAGTACATCAAC |
PCR Primers
| Primer | Sequence |
| SU | ACAGTTCGTGACCCATAGCC |
| SD | AGCAATAACTCCAAGCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16823A-C4 | VGB6 | C57BL/6NTac | |
| 16823A-F2 | VGB6 | C57BL/6NTac | |
| 16823A-F4 | VGB6 | C57BL/6NTac | |
| 16823A-F6 | VGB6 | C57BL/6NTac | |
| 16823A-H12 | VGB6 | C57BL/6NTac | |
