| Gene | Wfs1 |
| Chromosome | 5 |
| Deletion Start | 36,982,289 |
| Deletion End | 36,966,844 |
| Deletion Size | 15,446 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,981,984 | 36,982,004 | GCGGGCACATAGACCAGAATC |
| TUP | 36,981,956 | 36,981,980 | CAGAAGCTCCTCAAGTCGGGCTGCT |
| TUR | 36,981,927 | 36,981,947 | AAGTGGCAGCCTTGGTATCAG |
| TDF | 36,967,590 | 36,967,611 | CCTGCTCTTCTGCTGGTTCTAC |
| TDP | 36,967,562 | 36,967,588 | TGTACCGCTCAGAAGGCATGAAGGTCT |
| TDR | 36,967,537 | 36,967,554 | TGCTGCCAGGTGAGTGTG |
PCR Primers
| Primer | Sequence |
| SU | GACCAGCCTAAGCCTCTGTG |
| SD | GTAAGGAAAGGGCAGGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16832A-E4 | VGB6 | C57BL/6NTac | |
| 16832A-G1 | VGB6 | C57BL/6NTac | |
| 16832A-H5 | VGB6 | C57BL/6NTac | |
| 16832A-H7 | VGB6 | C57BL/6NTac | |
| 16832A-F7 | VGB6 | C57BL/6NTac | |
