| Gene | Adprh |
| Chromosome | 16 |
| Deletion Start | 38,450,438 |
| Deletion End | 38,445,667 |
| Deletion Size | 4,772 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,450,298 | 38,450,316 | GGCCCAGATGGGTGACTTG |
| TUP | 38,450,267 | 38,450,293 | CCATAGATGTGGCACGATGGAGAGTCA |
| TUR | 38,450,242 | 38,450,261 | GCCAGGTGCATGACGGTATC |
| TDF | 38,446,029 | 38,446,047 | CAACTCGGCTCCCGTCTTC |
| TDP | 38,446,004 | 38,446,027 | CGCAGCCCTTTGGTGTGAAGGAAA |
| TDR | 38,445,977 | 38,445,998 | AGCTCACGTCGATGTAGAACTG |
PCR Primers
| Primer | Sequence |
| SU | CTCGAGAGACCACGACACAG |
| SD | CTTGGGATAGGTGGGAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16836A-G10 | VGB6 | C57BL/6NTac | |
| 16836A-C9 | VGB6 | C57BL/6NTac | |
| 16836A-D4 | VGB6 | C57BL/6NTac | |
| 16836A-F3 | VGB6 | C57BL/6NTac | |
| 16836A-A10 | VGB6 | C57BL/6NTac | |
