| Gene | Pcdh12 |
| Chromosome | 18 |
| Deletion Start | 38,284,067 |
| Deletion End | 38,268,739 |
| Deletion Size | 15,329 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,283,866 | 38,283,886 | CAGGAGATGCCTTCCAGATTC |
| TUP | 38,283,840 | 38,283,862 | AGCTGCCTCAGGCACTGCCGGTT |
| TUR | 38,283,816 | 38,283,834 | CAGGCCGTCCTCAGAGTTC |
| TDF | 38,269,484 | 38,269,501 | TCCCTGGTGCTGGTGTTC |
| TDP | 38,269,460 | 38,269,481 | CCGTGCCAGGCTGAAACAGGCT |
| TDR | 38,269,436 | 38,269,455 | GGAGGCATGAGGACTGGTAG |
PCR Primers
| Primer | Sequence |
| SU | GCCTCATCCTCTTCAGCAAC |
| SD | TTCATGCCAGGAATTTGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16838A-H2 | VGB6 | C57BL/6NTac | |
| 16838A-B6 | VGB6 | C57BL/6NTac | |
| 16838A-F1 | VGB6 | C57BL/6NTac | |
