| Gene | Arih2 |
| Chromosome | 9 |
| Deletion Start | 108,644,274 |
| Deletion End | 108,611,637 |
| Deletion Size | 32,638 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,643,562 | 108,643,586 | CTGCTGTGATGAAATGATGTGTATC |
| TUP | 108,643,514 | 108,643,543 | ACAACTGTTTGATAGTACCCAGATGCCCTG |
| TUR | 108,643,469 | 108,643,490 | GAACCACCAGTACACCTGAAAC |
| TDF | 108,612,003 | 108,612,023 | GGGCTTCGTTTGACTCCTTTC |
| TDP | 108,611,964 | 108,611,992 | AGTACCGTCCAAGAGGTTTAAGGAGACAC |
| TDR | 108,611,941 | 108,611,960 | TCTCAACGGACTCCCACAAG |
PCR Primers
| Primer | Sequence |
| SU | TTCAGGACTTAGAGGCAGATG |
| SD | AGCACCCATGACCAGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16845A-E5 | VGB6 | C57BL/6NTac | |
| 16845A-G2 | VGB6 | C57BL/6NTac | |
| 16845A-E4 | VGB6 | C57BL/6NTac | |
