| Gene | Rwdd4a |
| Chromosome | 8 |
| Deletion Start | 47,550,767 |
| Deletion End | 47,533,923 |
| Deletion Size | 16,845 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,534,050 | 47,534,068 | TGTCCCACGTTGGTGGTTC |
| TUP | 47,534,069 | 47,534,091 | CCCTGCCAGATAGCACTGGGCAA |
| TUR | 47,534,117 | 47,534,137 | GGAGGCTGAGTCTCACGTAAC |
| TDF | 47,550,096 | 47,550,115 | GCCAAGGCCAACAGAGTCAG |
| TDP | 47,550,117 | 47,550,139 | CTGCCTCGGGCGGGAAGACATTC |
| TDR | 47,550,160 | 47,550,184 | GACCCGAGATACAGTTGCCATAAAG |
PCR Primers
| Primer | Sequence |
| SU | TCAGTAACTGGTCAGAGGTCT |
| SD | GACTCAGCACTGGGCTCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16852A-D8 | VGB6 | C57BL/6NTac | |
| 16852A-G4 | VGB6 | C57BL/6NTac | |
