| Gene | Tmem41a |
| Chromosome | 16 |
| Deletion Start | 21,947,483 |
| Deletion End | 21,934,822 |
| Deletion Size | 12,662 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,947,217 | 21,947,239 | TCTCCTTCCTCAACCCAATTACC |
| TUP | 21,947,192 | 21,947,216 | ACAGCAGCCAGTCTTCAGAGGTGCA |
| TUR | 21,947,159 | 21,947,180 | CTGGATCATAGCCTCGTCACTC |
| TDF | 21,935,377 | 21,935,395 | TGTTTGGCTCGTGGGTCTC |
| TDP | 21,935,347 | 21,935,375 | CCTTAAGCAGGTATGCAGACTTCAGCAGT |
| TDR | 21,935,298 | 21,935,318 | GACGGCAAGGTCATGTCTCAC |
PCR Primers
| Primer | Sequence |
| SU | GTCAGTCTCACATTTGTTGGC |
| SD | TGCTGCGTGTAAGACCTACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16854A-H8 | VGB6 | C57BL/6NTac | |
| 16854A-C12 | VGB6 | C57BL/6NTac | |
