| Gene | Liph |
| Chromosome | 16 |
| Deletion Start | 21,995,366 |
| Deletion End | 21,967,989 |
| Deletion Size | 27,378 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,994,467 | 21,994,486 | CCTGGCTGCCCAGAACTTAC |
| TUP | 21,994,435 | 21,994,461 | AAACCAGGCCAGTCTTGAACTCACTGG |
| TUR | 21,994,406 | 21,994,425 | GGCTGGGTGTAGTGGTGAAC |
| TDF | 21,968,783 | 21,968,804 | CCACTTGTCTCTGCATCTCCAG |
| TDP | 21,968,757 | 21,968,780 | CAGGGTCACAGACCCATGGTGCTG |
| TDR | 21,968,701 | 21,968,721 | TGGCTCCATACAAGCGTGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCTCTGTGGAGACCTTGGAG |
| SD | GACTACAGTGGTTTCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16859A-C4 | VGB6 | C57BL/6NTac | |
| 16859A-C5 | VGB6 | C57BL/6NTac | |
| 16859A-C10 | VGB6 | C57BL/6NTac | |
| 16859A-G3 | VGB6 | C57BL/6NTac | |
| 16859A-B5 | VGB6 | C57BL/6NTac | |
