| Gene | Crygc |
| Chromosome | 1 |
| Deletion Start | 65,073,502 |
| Deletion End | 65,071,511 |
| Deletion Size | 1,992 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,073,026 | 65,073,049 | GGTGTTCAAATGCCTGCCTAAATC |
| TUP | 65,072,999 | 65,073,023 | TGGGGATACAGACAGGAAGACAGAT |
| TUR | 65,072,966 | 65,072,988 | CTGGACCTTCTGTAAGTGGATTC |
| TDF | 65,071,719 | 65,071,739 | GGCTGCTGGGTTCTCTATGAG |
| TDP | 65,071,695 | 65,071,718 | ATGCCTAACTACCGAGGCCGCCAG |
| TDR | 65,071,628 | 65,071,648 | AGCCCGCCTTAGCATCTACAG |
PCR Primers
| Primer | Sequence |
| SU | TTTGCAGAATTCCAGCCTTC |
| SD | CTGCTTCCTCTCCAATAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16861A-G6 | VGB6 | C57BL/6NTac | |
| 16861A-G11 | VGB6 | C57BL/6NTac | |
| 16861A-H3 | VGB6 | C57BL/6NTac | |
| 16861A-H4 | VGB6 | C57BL/6NTac | |
| 16861A-H5 | VGB6 | C57BL/6NTac | |
| 16861A-D8 | VGB6 | C57BL/6NTac | |
