| Gene | 1810043H04Rik |
| Chromosome | 11 |
| Deletion Start | 120,100,263 |
| Deletion End | 120,098,985 |
| Deletion Size | 1,279 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,099,392 | 120,099,412 | CCCAGCTCCACTTGGTTTCTC |
| TUP | 120,099,414 | 120,099,439 | CAGCAATTCCTGCTAGATGCCAGGGC |
| TUR | 120,099,484 | 120,099,503 | GCGGACCTTGCACTTTACTC |
| TDF | 120,099,695 | 120,099,717 | AGCCCAGCCTCACCTACATACAG |
| TDP | 120,099,718 | 120,099,740 | CCTTTGGTTTCCCGGCAGAGGCT |
| TDR | 120,099,741 | 120,099,761 | CGCAACACCTGACGACTGAAC |
PCR Primers
| Primer | Sequence |
| SU | AGGTAACCTCCCAAACTGCC |
| SD | TTCTATGGTCCAGACCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16869A-D1 | VGB6 | C57BL/6NTac | |
| 16869A-D7 | VGB6 | C57BL/6NTac | |
| 16869A-H10 | VGB6 | C57BL/6NTac | |
| 16869A-H6 | VGB6 | C57BL/6NTac | |
| 16869A-G8 | VGB6 | C57BL/6NTac | |
| 16869A-G5 | VGB6 | C57BL/6NTac | |
