| Gene | 2310014L17Rik |
| Chromosome | 7 |
| Deletion Start | 12,928,924 |
| Deletion End | 12,927,899 |
| Deletion Size | 1,026 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,928,084 | 12,928,105 | TCTTATCTGCGTGTCACCCTTC |
| TUP | 12,928,108 | 12,928,133 | CGGCATTTTCAAGCTGCCTAAACGCC |
| TUR | 12,928,135 | 12,928,155 | CAGAATACGTGACCGCAGTCC |
| TDF | 12,928,763 | 12,928,785 | GCTACCAAAGACCCAGTAGAGAC |
| TDP | 12,928,793 | 12,928,822 | ATCCCGGATTTACCACCAGATCAAACACCA |
| TDR | 12,928,852 | 12,928,870 | CCCATCAGCCTGAGCCTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTCTCAGCTATTGGGCACC |
| SD | CCTTCCATCAAGACCAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16877A-D8 | VGB6 | C57BL/6NTac | |
| 16877A-D9 | VGB6 | C57BL/6NTac | |
| 16877A-F2 | VGB6 | C57BL/6NTac | |
| 16877A-G2 | VGB6 | C57BL/6NTac | |
