| Gene | Trim60 |
| Chromosome | 8 |
| Deletion Start | 65,001,592 |
| Deletion End | 65,000,135 |
| Deletion Size | 1,458 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,000,946 | 65,000,969 | GCGGAGTTTCTCAGAACTGAATGG |
| TUP | 65,000,912 | 65,000,940 | TTGAGACCCTGTTAGACCATGTTTCCACA |
| TUR | 65,000,882 | 65,000,902 | GGCCTCCACTTCCTTCAACAG |
| TDF | 65,000,463 | 65,000,484 | GCAATCAGTCCTCAGGTTTCTG |
| TDP | 65,000,428 | 65,000,456 | TGGGCGGTATGCTGAGAATAGTTATGTCA |
| TDR | 65,000,406 | 65,000,427 | TCCGTTCTTAGAGGTCCATAGG |
PCR Primers
| Primer | Sequence |
| SU | TTTCTCATCCACAGGAACCC |
| SD | CCTAAATAGGGCAGCAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16879A-A1 | VGB6 | C57BL/6NTac | |
| 16879A-B6 | VGB6 | C57BL/6NTac | |
| 16879A-C10 | VGB6 | C57BL/6NTac | |
| 16879A-E12 | VGB6 | C57BL/6NTac | |
| 16879A-F5 | VGB6 | C57BL/6NTac | |
