| Gene | Gpr55 |
| Chromosome | 1 |
| Deletion Start | 85,941,854 |
| Deletion End | 85,940,793 |
| Deletion Size | 1,062 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,941,750 | 85,941,769 | TCCTCCTTGGCCTGGTTCTC |
| TUP | 85,941,725 | 85,941,747 | CCTACTGGCCATCCGAGGCTTCA |
| TUR | 85,941,688 | 85,941,711 | GCGATATAGTCCAGCTTCCTCTTC |
| TDF | 85,941,114 | 85,941,137 | GTCATCTTTGTGGTCTCCTTTCTC |
| TDP | 85,941,088 | 85,941,109 | TGCACTTGGGCTTCTTCCTGCA |
| TDR | 85,941,032 | 85,941,052 | AGCTGATGCCCTGCTTCATTC |
PCR Primers
| Primer | Sequence |
| SU | AGCTGTCTCCCTCAATCACC |
| SD | AATCGCCAAGAGAGCTTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16895B-D1 | VGB6 | C57BL/6NTac | |
| 16895C-H9 | VGB6 | C57BL/6NTac | |
| 16895C-F12 | VGB6 | C57BL/6NTac | |
