| Gene | 6720456B07Rik |
| Chromosome | 6 |
| Deletion Start | 113,616,178 |
| Deletion End | 113,604,782 |
| Deletion Size | 11,397 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,605,346 | 113,605,366 | TGCTGAGGGTTGTAAGGGATG |
| TUP | 113,605,370 | 113,605,389 | CCCGCTCGGGCCTTGGGAAT |
| TUR | 113,605,435 | 113,605,454 | TCCGAGGAGCCAGACAGAAG |
| TDF | 113,615,907 | 113,615,927 | ACGCGGTGCAGAGGTTAAGTG |
| TDP | 113,615,928 | 113,615,953 | CTCTGCCTGGATGTAGCTGTCTGGGA |
| TDR | 113,615,954 | 113,615,974 | GTAGCAAAGGCAGCTTCAGAG |
PCR Primers
| Primer | Sequence |
| SU | GAGAGCTTGGAGCAACCC |
| SD | GCACAAGTCTCAGCCAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16901A-A10 | VGB6 | C57BL/6NTac | |
| 16901A-H12 | VGB6 | C57BL/6NTac | |
| 16901A-C4 | VGB6 | C57BL/6NTac | |
| 16901A-F10 | VGB6 | C57BL/6NTac | |
| 16901A-D11 | VGB6 | C57BL/6NTac | |
| 16901A-F2 | VGB6 | C57BL/6NTac | |
