| Gene | Fam134a |
| Chromosome | 1 |
| Deletion Start | 75,147,155 |
| Deletion End | 75,142,866 |
| Deletion Size | 4,290 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,143,334 | 75,143,354 | TTGGGAGCCTCAACTGAGATG |
| TUP | 75,143,357 | 75,143,384 | TGTCTCCCTAAGGTTGTTGTCTTCGTCG |
| TUR | 75,143,396 | 75,143,417 | TGAGATGCTGAGCAGGAAGAAG |
| TDF | 75,146,670 | 75,146,690 | GAGTGAAATGCCCTCCTGGTG |
| TDP | 75,146,691 | 75,146,713 | CACCAGTGAAGACCCTGAGCCCA |
| TDR | 75,146,732 | 75,146,752 | GGTGCTGGATGGAGCCATTAG |
PCR Primers
| Primer | Sequence |
| SU | CCTCGCAGCCTCAACGCC |
| SD | ATAAAGCCAACGGGAGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16913A-F4 | VGB6 | C57BL/6NTac | |
| 16913A-H8 | VGB6 | C57BL/6NTac | |
