| Gene | Ino80c |
| Chromosome | 18 |
| Deletion Start | 24,121,798 |
| Deletion End | 24,106,427 |
| Deletion Size | 15,372 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,121,309 | 24,121,332 | GAGAAGTTTGCGGGTCATCCATAG |
| TUP | 24,121,285 | 24,121,308 | CGCATCTCACAGACACCCTGTGGG |
| TUR | 24,121,216 | 24,121,238 | TGAAGGCAACCACTGGTATCAAC |
| TDF | 24,106,936 | 24,106,959 | AGCGTTCAGCCGTCACTTATTCTC |
| TDP | 24,106,908 | 24,106,933 | CACTCTGACCAGCCAGGAGTATCCGC |
| TDR | 24,106,884 | 24,106,903 | TCCCTGCCTTCTAACTCCAC |
PCR Primers
| Primer | Sequence |
| SU | TCAGCAATCTGTTCGTGGAC |
| SD | CGCTTGTTAGGCTCCAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16927A-B5 | VGB6 | C57BL/6NTac | |
| 16927A-F4 | VGB6 | C57BL/6NTac | |
| 16927A-E12 | VGB6 | C57BL/6NTac | |
| 16927A-D10 | VGB6 | C57BL/6NTac | |
| 16927A-C7 | VGB6 | C57BL/6NTac | |
| 16927A-C3 | VGB6 | C57BL/6NTac | |
