| Gene | Gpr89 |
| Chromosome | 3 |
| Deletion Start | 96,905,220 |
| Deletion End | 96,871,376 |
| Deletion Size | 33,845 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,904,851 | 96,904,869 | CGCGAGGTCCAAGTCTGAG |
| TUP | 96,904,819 | 96,904,847 | TCCTTTGTGCAGTGACACCTCATCTTTCG |
| TUR | 96,904,787 | 96,904,807 | CGACCCGCAAAGAGTGAACAG |
| TDF | 96,871,466 | 96,871,489 | CACCGTTGGTTTGATGTCATCTTC |
| TDP | 96,871,444 | 96,871,465 | CTGGTCAGTGCCCTCTCCAGCA |
| TDR | 96,871,402 | 96,871,421 | GCTTCTCTGGTGCCTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGGACCTCGCACAACTCTC |
| SD | CAAGCTGTGCTGGACAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16941B-D4 | VGB6 | C57BL/6NTac | |
| 16941B-F7 | VGB6 | C57BL/6NTac | |
| 16941C-E7 | VGB6 | C57BL/6NTac | |
| 16941C-C5 | VGB6 | C57BL/6NTac | |
| 16941C-C4 | VGB6 | C57BL/6NTac | |
