| Gene | Plxdc2 |
| Chromosome | 2 |
| Deletion Start | 16,357,233 |
| Deletion End | 16,357,129 |
| Deletion Size | 105 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,357,131 | 16,357,148 | AAGGTTCCGGAGGGCCGA |
| TUP | 16,357,149 | 16,357,171 | CCTGGCCGCAGCAGGAGTTATGT |
| TUR | 16,357,197 | 16,357,214 | CCGTGGGCGAACTGGAAC |
| TDF | 16,357,150 | 16,357,167 | CTGGCCGCAGCAGGAGTT |
| TDP | 16,357,186 | 16,357,211 | TTAACAGACCGGTTCCAGTTCGCCCA |
| TDR | 16,357,213 | 16,357,231 | TATGGTGTCCAGGCTCCCC |
PCR Primers
| Primer | Sequence |
| SU | CTTGGTTGATTGTGTCTCAG |
| SD | GGTGGAGTAAGCATCATAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16990A-G6 | VGB6 | C57BL/6NTac | |
| 16990B-A8 | VGB6 | C57BL/6NTac | |
| 16990B-A12 | VGB6 | C57BL/6NTac | |
| 16990B-C4 | VGB6 | C57BL/6NTac | |
| 16990B-F1 | VGB6 | C57BL/6NTac | |
| 16990B-H3 | VGB6 | C57BL/6NTac | |
