| Gene | Kcnip2 |
| Chromosome | 19 |
| Deletion Start | 45,815,800 |
| Deletion End | 45,793,634 |
| Deletion Size | 22,167 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,815,774 | 45,815,794 | CAAGGCCGAAAGGAGAGTTTG |
| TUP | 45,815,750 | 45,815,772 | CCGAATCCCGAGATTTGGACGGC |
| TUR | 45,815,717 | 45,815,737 | AGCCACCTACTCACCCGTAAG |
| TDF | 45,794,066 | 45,794,088 | CTCCTGCCTGACTACCTTCTTAC |
| TDP | 45,794,031 | 45,794,055 | CAGAAACAAGGACGGCGTGGTGACC |
| TDR | 45,793,990 | 45,794,011 | TGGAGCCATACCTGTTGACAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTCCGAGCTGCAGAG |
| SD | GGAAAGCACCATAGCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16998A-A12 | VGB6 | C57BL/6NTac | |
| 16998A-D8 | VGB6 | C57BL/6NTac | |
| 16998A-G3 | VGB6 | C57BL/6NTac | |
| 16998A-F7 | VGB6 | C57BL/6NTac | |
