| Gene | N4bp2l1 |
| Chromosome | 5 |
| Deletion Start | 150,594,433 |
| Deletion End | 150,572,677 |
| Deletion Size | 21,757 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 150,593,574 | 150,593,594 | CCTGCCCTGCACTTGGTATTC |
| TUP | 150,593,540 | 150,593,567 | CGTTGGACTGTTTCTCTCTCCTTGCTGG |
| TUR | 150,593,491 | 150,593,511 | CAAAGGGTGACGGTGGGAAAG |
| TDF | 150,573,172 | 150,573,190 | GCAGGGCAGCATGAGAAAC |
| TDP | 150,573,147 | 150,573,171 | CGCAGCAGAGCTGTCGAAGTACCTT |
| TDR | 150,573,110 | 150,573,130 | CCTCAGGAGACCAGGCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTCCACAGTGACTGCAG |
| SD | GTGGAGAGGCTGGATCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17004A-B1 | VGB6 | C57BL/6NTac | |
| 17004A-H7 | VGB6 | C57BL/6NTac | |
| 17004A-C8 | VGB6 | C57BL/6NTac | |
| 17004A-C1 | VGB6 | C57BL/6NTac | |
| 17004A-H5 | VGB6 | C57BL/6NTac | |
