| Gene | Hoxa9 |
| Chromosome | 6 |
| Deletion Start | 52,226,101 |
| Deletion End | 52,224,183 |
| Deletion Size | 1,919 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,225,452 | 52,225,469 | CAGCGCGTCAGCTTTCTC |
| TUP | 52,225,431 | 52,225,451 | TGGAGCCTGCCTTCGTCCTCG |
| TUR | 52,225,383 | 52,225,405 | CCAAACACCAACTTCTGGTTCTC |
| TDF | 52,224,638 | 52,224,659 | TGAGACTTTCTTGGCTCTGTTG |
| TDP | 52,224,610 | 52,224,635 | AGTGACCCTCCGTGGATCTGTCTGCC |
| TDR | 52,224,569 | 52,224,589 | GGATGCCGCTGAATTGGTTAG |
PCR Primers
| Primer | Sequence |
| SU | GAGTACAGAGGCAAGGCCAG |
| SD | GAATGATCTGCCACACGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17012A-H12 | VGB6 | C57BL/6NTac | |
| 17012A-F4 | VGB6 | C57BL/6NTac | |
| 17012A-C6 | VGB6 | C57BL/6NTac | |
| 17012A-A8 | VGB6 | C57BL/6NTac | |
