| Gene | Mpp3 |
| Chromosome | 11 |
| Deletion Start | 102,025,753 |
| Deletion End | 102,000,444 |
| Deletion Size | 25,310 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,025,505 | 102,025,527 | GGACCTTGTTTCTCTGGCTCTTC |
| TUP | 102,025,480 | 102,025,504 | CTCAGGTTTGCATGAGACCCTGGCA |
| TUR | 102,025,427 | 102,025,446 | AAGCCCATCTCCTCCCTGTG |
| TDF | 102,001,262 | 102,001,281 | GGGTCCACATCCTTGCACAG |
| TDP | 102,001,229 | 102,001,258 | ACACCACCCTAATGAACCATCTTCTCAGCC |
| TDR | 102,001,206 | 102,001,227 | TGGCTGCTAACATCACAAAGAG |
PCR Primers
| Primer | Sequence |
| SU | TTAGGGATGCCCAGTCTCTG |
| SD | AGCTGGACCAGTGAAGTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17015A-C1 | VGB6 | C57BL/6NTac | |
| 17015A-C2 | VGB6 | C57BL/6NTac | |
