| Gene | Kcnd1 |
| Chromosome | X |
| Deletion Start | 7,836,525 |
| Deletion End | 7,823,846 |
| Deletion Size | 12,680 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,824,615 | 7,824,634 | GCTTCCTGCGGAGTGTGATG |
| TUP | 7,824,635 | 7,824,659 | AGCCTTATCGATGTGGTGGCCATCC |
| TUR | 7,824,664 | 7,824,687 | GGCACGAACAGCCCAATATAGTAG |
| TDF | 7,836,144 | 7,836,165 | GCAGGGAGAATTTGTAGCACAG |
| TDP | 7,836,168 | 7,836,193 | CAAGGGACCCAGTCGGTGCTTAATGA |
| TDR | 7,836,269 | 7,836,289 | GGCTTGGCATTGAGGCTTGAG |
PCR Primers
| Primer | Sequence |
| SU | TTCCATCTCCACCGCTCTG |
| SD | AATTGGAGATGAGGGTTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 146 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17025A-C3 | VGB6 | C57BL/6NTac | |
| 17025A-D5 | VGB6 | C57BL/6NTac | |
| 17025A-E3 | VGB6 | C57BL/6NTac | |
| 17025A-F11 | VGB6 | C57BL/6NTac | |
| 17025A-G2 | VGB6 | C57BL/6NTac | |
| 17025A-H7 | VGB6 | C57BL/6NTac | |
