| Gene | Pim2 |
| Chromosome | X |
| Deletion Start | 7,882,524 |
| Deletion End | 7,878,322 |
| Deletion Size | 4,203 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,878,746 | 7,878,763 | GGCTTTGGCACCGTCTTC |
| TUP | 7,878,765 | 7,878,785 | CGGGACACCGCGTCACGGATA |
| TUR | 7,878,810 | 7,878,831 | AAACACCTGACCCTCCGAGAAC |
| TDF | 7,882,275 | 7,882,296 | AGCCCAACCGGCTTCTTTCTTC |
| TDP | 7,882,298 | 7,882,319 | CAAGCCTCCCAAGCCTCGCTGA |
| TDR | 7,882,358 | 7,882,377 | ACCGGCGGATTAGGGCACAG |
PCR Primers
| Primer | Sequence |
| SU | ATCCTAGGAAAGGAGGCTGC |
| SD | GGAAGTGCCAGTGTAGGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17026A-C4 | VGB6 | C57BL/6NTac | |
| 17026A-C7 | VGB6 | C57BL/6NTac | |
| 17026A-D10 | VGB6 | C57BL/6NTac | |
| 17026A-H2 | VGB6 | C57BL/6NTac | |
| 17026A-A4 | VGB6 | C57BL/6NTac | |
| 17026A-A5 | VGB6 | C57BL/6NTac | |
